[关键词]
[摘要]
目的研究贯叶连翘提取物体内外对甲1型流感病毒(IAV)活性的影响。方法以细胞存活率和抑制率为指标,采用MTT比色法检测贯叶连翘提取物体外抑制IAV活性的效应;体内实验以流感病毒滴鼻感染小鼠,分别ig给药,观察小鼠死亡率、生命延长率及小鼠病毒性肺炎的改变,判定其抗流感病毒作用。结果贯叶连翘提取物在体外随药物质量浓度的增加其细胞存活率和病毒抑制率明显升高,与病毒对照组相比均有显著性差异(P<0.01);并可降低流感病毒感染小鼠的死亡率,延长存活时间,明显减轻小鼠病毒性肺炎的症状(P<0.01)。结论贯叶连翘提取物在体内外均有良好的抗IAV活性的作用。
[Key word]
[Abstract]
Objective To study the antiviral effect of Hypericum perforatum extract(HPE) on influenza A virus(IAV) in vitro and in vivo.Methods Cell survival rate and inhibition rate were served as target to evaluate antiviral activity of HPE by MTT.Kunming mice infected with intranasal IAV were treated by ig HPE and Oseltamivir in vivo,respectively.The major criteria for evaluating the effects of HPE on mortality,life-prolong rate,and viral pneumonia of mice.Results The results showed that cell survival rate and inhibitory rate increased with the increasing concentration of HPE in vitro. There were significant differences as compared with the virus control group(P0.01).HPE could decrease the mortality of mice infected with IAV,prolong survival time,and reduce that symptoms of viral pneumonia(P0.01).Conclusion These results indicates that HPE showed an obvious antiviral activity on IAV in vitro and in vivo.
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[基金项目]
中国科学院“百人计划”资助项目(2008-287)