[关键词]
[摘要]
目的研究大孔吸附树脂分离纯化川西獐牙菜中环烯醚萜苷类和酮类成分的工艺。方法考察了HPD-300、HPD-400、HPD-600、AB-8、DM-301及D-101-Ⅰ等6种吸附树脂对环烯醚萜苷类和酮类的指标成分獐牙菜苦苷和当药醇苷的动态吸附及洗脱性能,从中筛选出效果较好的树脂完成实验研究。结果HPD-300用于同时分离纯化环烯醚萜苷类和酮类成分,吸附洗脱性能良好,其吸附过程上样量为0.9 g生药/mL树脂,上柱液pH值约为8,体积流量为2 BV/h;洗脱过程采用水洗除杂,7 BV 20%、5BV 70%乙醇梯度洗脱,洗脱率都在90%以上,所得2个固体样品中两类成分质量分数均可达50%以上。结论HPD-300用于同时富集环烯醚萜苷类和酮类成分效果最佳,是一种理想的分离纯化介质。
[Key word]
[Abstract]
Objective To study the separation and purification technology of iridoid glycosides and xanthones from Swertia mussotii by macroporous resin.Methods The dynamic adsorption and desorption characteristics of macroporous adsorption resins HPD-300,HPD-400,HPD-600,AB-8,DM-301,and D-101-Ⅰ for swertiamarin and swertianolin were investigated.On the base of the investigation,the better macroporous adsorption resin was chosen to finish the experiments.Results The adsorption and elution characteristics of HPD-300 used to separate and purify iridoid glycosides and xanthones were very good.In the course of adsorption,the amount of used adsorption is 0.9 g dried medicinal herb/mL resin,with about 8 pH value of adsorption solution,and the volumetric rate is 2 BV/h; In the course of elution,the resin column chromatography was eluted gradiently with 7 BV 20% and 5 BV 70% EtOH after removing impurities with water.The elution rates were both more than 90%,and the contents of iridoid glycosides and xanthones were both up to 50% in the two dried fractions of 20% and 70% ethanolic elutions.Conclusion HPD-300 is an ideal resin with the best enrichment for separating and purifying both iridoid glucosioles and xanthanoes simultanously.
[中图分类号]
[基金项目]
天津市应用基础及前沿技术研究计划(08JCZDJC24700)