目的研究泻心汤对高脂饮食联合注射链脲佐菌素(STZ)诱导的糖尿病大鼠早期肾病的影响。方法采用高脂饮食并注射低剂量链脲佐菌素方法复制糖尿病大鼠模型, 随机分为模型组、泻心汤组及二甲双胍组, 并设对照组连续给药13周后, 检测各组血糖(FBG)、糖化血红蛋白(HbAlc)、血脂(TG和TC)、血清胰岛素(INS)、肾功能、肾脏指数及尿蛋白, 光镜、电镜下观察肾脏组织结构的变化。结果与模型组大鼠相比较, 泻心汤给药13周明显缓解大鼠多食、多饮、多尿症状, 降低HbAlc、胰岛素抵抗指数(IRI)、尿蛋白排泄率、肌酐清除率(CCr)和血脂水平, 改善肾小球基底膜增厚及足突融合变化。结论泻心汤有抗实验性糖尿病大鼠早期肾病的作用, 作用机制可能与其降低血脂和HbAlc及改善胰岛素抵抗等作用相关。

Objective To investigate the effect of Xiexin Decoction(XXD) on the early diabetic nephropathy(DN) of diabetic rats induced by high-fat diet with injection of streptozotocin(STZ).MethodsMale Sprague-Dawley rats were divided into two groups and fed with normal pellet diet(NPD) or high-fat diet(HFD),respectively,for a period of four weeks and then HFD-fed rats were ip injected with STZ.The diabetic rats were divided into three groups: model group,XXD group,and metformin group.After 13-weeks ig administration,fasting blood glucose(FBG),glycated hemoglobin(HbAlc),blood lipids(TG and TC),serum insulin(INS),renal function,kidney index,albumin in urine,and the renal histology and ultrastructure were observed.Results Compared with the model group,XXD reduced the levels of water intake,food consumption,urine volume,HbAlc,insulin resistance index(IRI),creatinine clearance rate(CCr),albumin in urine and blood lipids(P0.05 or P0.01) and decreased the incrassation of the glomerular basement membrane,and the change of foot process fusion.Conclusion XXD could protect the kidney in rats with experimental early DN.The nephroprotection of XXD may be related to the decrease in blood lipids and HbAlc and improvement in insulin resistance.

国家自然科学基金资助项目 (30873231);上海市科委基金资助项目 (09DZ1971700);上海市教委基金资助项目 (09ZZ129)