目的研究北葶苈子Lepidium apetalum的化学成分。方法采用硅胶, 聚酰胺、反相硅胶C₁₈和Sepha-dex LH-20柱色谱对北葶苈子75%乙醇提取物进行了分离纯化。用质谱、一维及二维核磁等波谱学方法确定了化合物结构。结果从北葶苈子中分离得到个化合物, 分别鉴定为槲皮素3--O-β-D-[2-O-(6-O-芥子酰基)-β-D-吡喃葡萄糖基]-吡喃葡萄糖苷(1)、异鼠李素3--O-β-D-[2-O-(6-O-芥子酰基)-β-D-吡喃葡萄糖基]-吡喃葡萄糖苷(2)、槲皮素-7-O-β-D-吡喃葡萄糖苷(3)、槲皮素-3-O-β-D-吡喃葡萄糖苷(4)、异鼠李素-3-O-β-D-吡喃葡萄糖苷(5)、异鼠李素-7-O-β-D-吡喃葡萄糖苷(6)、山柰酚-7-O-β-D-吡喃葡萄糖苷(7)、槲皮素(8)、β-谷甾醇(9)、胡萝卜苷(10)、蔗糖(11)。结论所有化合物均为首次从该种植物中分离得到。其中化合物为首次从该属植物中分离得到, 化合物2为首次从十字花科植物中分离得到。

Objective To isolate the chemical constituents from the seeds of Lepidium apetalum and identify their structures.Methods The powdered seeds of L.apetalum were extracted with 70% ethanol and purified by polyamide,silica gel,RP-C18 and Sephadex LH-20 column chromatography.Chemical structures were identified by MS,1D,and 2D NMR experiments.Results Eleven compounds were isolated from the seeds of L.apetalum,they are quercetin-3-O-[2-O-(6-O-E-sinapoyl)-β-D-glucopymosyl]-β-D-glucopyranoside(1),isorhamnetin-3-O[2-O-(6-O-E-sinapoyl)-β-D-glucopymosyl]-β-D-glucopyranoside(2),quercetin-7-O-β-D-glucopyranoside(3),quercetin-3-O-β-D-glucopyranoside(4),isorhamnetin-3-Oβ-D-glucopyranoside(5),isorhamnetin-7-O-β-D-glucopyranoside(6),kaempferol-7-O-β-D-glucopyranoside(7),quercetin(8),β-sitosterol(9),daucosterol(10),and sucrose(11).Conclusion All these compounds are isolated from the seeds of L.apetalum firstly.Compounds 1 and 2 are isolated from the plants of Lepidium L.and Cruciferae plants for the first time,respectively.

国家自然科学基金资助项目 (30371776)