[关键词]
[摘要]
目的建立枳实药材HPLC指纹图谱分析方法。方法采用Diamonsil C18柱(250mm×4.6mm,5μm),以乙腈-0.01%磷酸二氢钠水溶液梯度洗脱,测定了36批枳实药材的指纹图谱。应用相似度分析、聚类分析和主成分分析,对枳实药材进行分类,建立枳实药材指纹图谱的共有模式,并应用主成分分析对共有模式进行研究。结果在选定的色谱条件下,得到两类枳实药材HPLC指纹图谱;根据相似度分析、聚类分析和主成分分析的结果,将36批药材分为2类,分别建立指纹图谱。结论应用本方法评价枳实药材的质量是可行的。
[Key word]
[Abstract]
Objective To establish HPLC fingerprint for Fructus Aurantii Immaturus.Methods The HPLC method was used with Diamonsil-C18 column (250 mm×4.6 mm,5 μm),and a mixture liquid of acetonitrile-0.01% NaH2PO4 as mobile phase in a gradient elution.The HPLC fingerprint for 36 batches of Fructus Aurantii Immaturus was studied on their similarity,cluster,and principal components analyses.The common HPLC fingerprint of Fructus Aurantii Immaturus was established,which was studied with principal components analyses.Results Under the selected spectrum condition,the 36 batches of Fructus Aurantii Immaturus were classified into two groups based on the result of similarity,cluster,and principal components analyses.Conclusion This method is reasonable and reliable to the quality control of Fructus Aurantii Immaturus.
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[基金项目]
“十一五”国家科技支撑计划项目(2006BAI06A01-02);2007天津市科技创新专项项目