[关键词]
[摘要]
目的研究生物因子(枯草芽孢杆菌)对凤仙花离体繁殖的影响。方法采用不同浓度枯草芽孢杆菌(0,5×104、5×105、5×106、5×107、5×108个/mL)处理凤仙花后,以其叶和茎为外植体,接种于MS+0.5mg/LNAA+0.5mg/L6-BA培养基上进行愈伤组织诱导和植株再生。结果无论是叶还是茎作外植体,经5×105个/mL浓度的菌液处理后愈伤组织诱导率最高(叶:95.3%,茎:13.3%),与对照差异极显著。经5×105个/mL浓度的菌液处理后的幼苗生长速度也最快,50d便达到了移栽要求。结论适当浓度的枯草芽孢杆菌处理有利于凤仙花的离体繁殖。
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[Abstract]
Objective To study if Bacillus subtilis can affect the rapid clonal propagation of Impatiens balsamina.Methods To treat I.balsamina by B.subtilis,at different concentration of 0.5×104,5×105,5×106,5×107,and 5×108 cfu/mL,then it was cultured on MS + 0.5 mg/L NAA + 0.5 mg/L 6-BA with leaves and stems as explants.Results The rate of callus induction was the highest by 5×105 cfu/mL,they were 95.3% (leaf as explant) and 13.3% (stem as explant),and analysis (t test) was remarkable.Meanwhile,young plant grew faster and better by 5×105 cfu/mL than other treatments,and could be transplanted to garden soil after 50 d.Conclusion B.subtilis is favorable for the rapid clonal propagation of I.balsamina
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[基金项目]
国家重点基础研究发展规划资助项目(G1999043508);安徽工程科技学院青年科研基金(2003YQ004);安徽工程科技学院青年教师科研资助项目(2008rzr004);安徽省高校省级自然科学研究项目(KJ2009B1232)