目的探讨藏药佐太对西红花苷-1在大鼠体内药动学特征的影响。方法对照组(连续ig生理盐水7d)和实验组(连续ig佐太混悬液7d或21d)大鼠按5mg/kg剂量im西红花苷-1后,采用反相高效液相色谱法测定给药后大鼠血浆中西红花苷-1的质量浓度,DAS2.0软件计算各组大鼠西红花苷-1的药动学参数。结果实验组大鼠分别按10mg/(kg.d)连续ig佐太7d和21d后,西红花苷-1的药动学特征发生明显变化,AUC、Cmax和MRT显著大于对照组,而CL和Vd显著小于对照组,并且随着佐太用药时间的延长,西红花苷-1的AUC增大。结论给予佐太后西红花苷-1在大鼠体内的吸收显著增多、消除显著减慢。

Objective To study the effect of Tibetan medicine Zuotai on in vivo pharmacokinetics of crocin-1 in rats.Methods After im injection of crocin-1 (5 mg/kg) in control (continuously ig normal saline for 7 d) and expermental (continuously ig Zuotai suspension for 7 d or 21 d) rats,The plasma concentration of crocin-1 was determined by RP-HPLC,and plasma concentration-time data were analyzed by DAS 2.0 software to get the related pharmacokinetic parameters of crocin-1.Results After continuously ig administration of Zuotai [10 mg/(kg·d)] for 7 d and 12 d in experimental rats,the pharmacokinetic parameters of crocin-1 changed significantly.The AUC,Cmax,and MRT were significantly greater in experimental rats than those in control rats,and the CL and Vd were significantly lower than those in control rats,and the AUC of crocin-1 was greater in the 21 d administration group than that in the 7 d administration group.Conclusion The result demonstrates that Tibetan medicine significantly affects the pharmacokinetics of crocin-1 in rats.After administration of Zuotai in rats,the absorption degree of crocin-1 is significantly increased and the clearance rate is significantly decreased.

国家中医药管理局中医药科学技术研究专项(国中管04-05ZM04)