目的考察吸收促进剂配伍对葛根素肠吸收的影响,并进一步观察复合吸收促进剂对肠黏膜的毒性。方法运用外翻肠囊法,采用L₉(3)⁴正交设计试验考察各吸收促进剂的协同作用,确定较理想复合吸收促进剂;运用显微形态学观察复合吸收促进剂对肠黏膜的毒性。结果复合吸收促进剂:1%壳聚糖、0.02%白芷挥发油、0.06%冰片配伍具有协同作用,可显著提高葛根素的肠吸收;复合吸收促进剂无肠黏膜毒性。结论1%壳聚糖、0.02%白芷挥发油、0.06%冰片配伍可安全有效地促进葛根素的肠吸收。

Objective To investigate the influence of the compatibility of absorption enhancers(AEs)on the intestinal absorption of puerarin and study the AEs' toxicities to the intestinal.Methods The in vitro everted intestinal sac was used as model.An orthogonal L₉(3)⁴ test design was used to optimize a best multi-combination of AEs and investigate the synergistic effect of the optimized AEs on intestinal permeability of puerarin.And the AEs' toxicities were further studied by light microscopy.Results The results showed that a multi-combination which including 1% chitosan,0.02% oil from Radix Angelicae,and 0.06% borneol had synergistic effect,which could significantly enhance the intestinal absorption of puerarin.And the multi-combination had no toxicities.Conclusion A multi-combination which including 1% chitosan,0.02% oil from Radix Angelicae Dahuricae,and 0.06% borneol could safely and efficiently enhance the intestinal absorption of puerarin.

江西省自然科学基金资助项目(2007GZY0932)