目的利用稻瘟霉模型快速筛选粗疣合叶苔内生真菌中具有生物活性的菌株,并对活性菌株T11进行鉴定。方法首次对粗疣合叶苔内生真菌进行了分离,通过观察内生真菌醋酸乙酯提取物引起稻瘟霉分生孢子或菌丝形态生长异常或生长抑制的情况,初步确定具有生物活性的菌株,进一步进行体外抗真菌及抗肿瘤活性筛选,对活性最好的T11菌株进行了分类鉴定。结果粗疣合叶苔分离得到47株内生真菌,具有抗稻瘟霉活性的活性菌株40株,占总分离菌株的85.1%。其中T11号菌株可明显抑制真菌生长和肿瘤细胞增殖。结论粗疣合叶苔内生真菌具有明显的抗真菌和细胞毒作用,是潜在的抗菌、抗肿瘤药物资源。

Objective To rapidly screen the bioactive strains in endophytic fungi from Scapania verrucosa by Pyricularia oryzae P-2b model and to identify the active strain T11.Methods The endophytic fungi were firstly isolated from S.verrucosa and the morphological deformation induced by ethyl acetate extract of bioactive fungi or growth inhibition of mycelia germinated from the conidia of P.oryzae were observed to screen the bioactive strains whose in vitro antifungus activity and cytotoxic effect on tumor cell lines were studied furtherly.The active strain T11 with the best activity was identified by morphological method.Results A total of 47 endophytic fungi were isolated from liverwort S.verrucosa.Among them,40 strains(85.1%)of endophytes could induce morphological abnormality of P.oryzae.The strain T11 could inhibit tumor cells proliferation and fungal mycelia growth effectively.Conclusion Endophytes from S.verrucosa have antifungal activity and cytotoxic effect and could be a potential resource for the development of antifungal and antitumor agent.

福建省自然科学基金资助项目(2008J0097)