目的采用多指标综合评价和SPSS软件分析,研究白花蛇舌草三效动态逆流提取工艺条件。方法以总黄酮、总多酚的量、清除DPPH自由基能力以及提取率为优化指标,采用正交实验设计,考察乙醇浓度、固液比、提取温度和提取时间对优化指标的影响。结果最佳提取工艺条件为:阶段提取时间为70min,固液比为1∶14,乙醇体积分数为50%,提取温度为85℃。结论实验室摸拟多效动态逆流提取白花蛇舌草的工艺研究可为企业大规模生产探索条件。

Objective To study the lab-scale simulated method of three-effect dynamic countercurrent extraction from Hedyotis diffusa by multi-index optimization and SPSS software.Methods Polyphenol content,flavonoids content,scavenging activity of samples on DPPH radicals,and extracting rate were considered as the optimization indexes.Orthogonal design method was used to optimize the four main factors of alcohol concentration,ratio of solid to solution,extracting temperature,and extracting times.Results The optimum condition is 70 min extraction time,solid to solution ratio of 1∶14,50% alcohol,extracting temperature 85 ℃.Conclusion The condition of large-scale industrialized production might be explored by studying the lab-scale simulated method of dynamic countercurrent extraction from H.diffusa.

国家“十一五”科技支撑计划(2006BAI06A18-06)