目的观察复方瑞康欣对吗啡依赖戒断大鼠焦虑行为的影响,并探讨其作用机制.方法健康雄性SD大鼠,剂量递增法sc吗啡10 d,确认吗啡依赖模型建立成功后,在吗啡自然戒断的1~3 d,复方瑞康欣100、200、300mg/kg和丁螺环酮15 mg/kg ig治疗(2次/d),于末次吗啡注射后72 h,高架十字迷宫实验评价大鼠的焦虑水平,免疫组化检测各组大鼠海马突触素的表达.结果与生理盐水(NS)治疗组比较,复方瑞康欣200、300 mg/kg和丁螺环酮治疗能明显增加大鼠进入开臂次数和时间的比例(P<0.01、0.05),同时海马突触素的表达显著降低(P<0.01).结论复方瑞康欣对吗啡依赖大鼠戒断后的焦虑治疗效果明显;逆转焦虑大鼠海马增高的突触素/突触,可能是复方瑞康欣临床治疗吗啡戒断后焦虑的机制之一.

Objective To observe effect of Compound Ruikangxin Capsula(CRC)on anxiety-like symptoms of morphine-withdrawal rats and investigate its mechanism.Methods The elevating doses were applied to validating anxiety-like behavior in SD male rats with sc morphine for 10 d.Treatments with ig administration of CRC(100,200,and 300 mg/kg)and buspirone(15 mg/kg)were performed during 1-3 d morphine withdrawal(twice every day).The plus elevated maze was applied to validating anxiety-like symptom in rats.The expression levels of synaptophysin were detected on hippocampus in groups by immunohistochemitry 72 h after scmorphine.Results As compared with the control group,animals treated with CRC(200 and 300 mg/kg)and buspirone had higher ratio of entry into open arm(P<0.01),as well as time of remaining in open arm(P <0.05).At that,lower level of synaptophysin was measured on hippocampus from those rats(P<0.01).Conclusion CRC would play an important role in rehabilitating the anxiety-like behavior in morphine-withdrawal rats.Reversal of synaptophysin on hippocampus of anxious rats could be contributed to exploring the mechanism.

国家自然科学基金资助项目(30860373);教育部科学技术重点项目(206136);