[关键词]
[摘要]
目的采用高效液相色谱法研究并建立了产于张家界的刺壳花椒药材的指纹图谱。方法Diamonsil C18色谱柱(250 mm×4.6 mm,5μm),乙腈-水流动相系统梯度洗脱,体积流量1.0 mL/min,检测波长278 nm。结果对所采集的10批刺壳花椒药材进行了测定,建立了刺壳花椒的指纹图谱,共有11个共有峰。结论HPLC法色谱指纹图谱分析技术可用于刺壳花椒药材质量的控制。
[Key word]
[Abstract]
Objective To study chromatography fingerprints of Zanthoxylum echinocarpum by HPLC.Methods Diamonsil C18 column(250 mm×4.6 mm,5 μm) was used with acetonitrile-water gradient elution,at flow-rate of 1.0 mL/min,detection wavelength at 278 nm.Results HPLC was used to establish fingerprint chromatograph of water extraction from ten batches of Z.echinocarpum,which had been harvested from the same area in Zhangjiajie at the same time.The result showed that 11 peaks were common.Conclusion The method could be used for the quality control of Z.echinocarpum.
[中图分类号]
[基金项目]
湖南省科学技术厅科技计划重点项目(2007SK2005)