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[摘要]
目的研究巴西人参Pfaffia paniculata根的化学成分,阐明其药效物质基础。方法利用薄层色谱、硅胶柱色谱、Sephadex LH-20凝胶色谱等进行分离纯化,利用波谱技术和理化常数鉴定化合物结构。结果分离得到12个化合物,分别鉴定为蜕皮甾酮(Ⅰ)、3-甲氧基-4-羟基-苯甲酸(Ⅱ)、齐墩果酸-3-O-β-D-葡萄糖醛酸苷(Ⅲ)、齐墩果酸-28-O-β-D-吡喃葡萄糖酯苷(Ⅳ)、齐墩果酸(Ⅴ)、豆甾醇-β-D-葡萄糖苷(Ⅵ)、豆甾醇(Ⅶ)、胡萝卜苷(Ⅷ)、β-谷甾醇(Ⅸ)2、0,22-异丙亚二氧基-β-蜕皮激素(Ⅹ)、1-O-β-D-吡喃葡萄糖基-(2S,3S,4R)-2-[2′R-羟基-十六碳酰胺]-十八碳-4-烯-1,3-二醇(Ⅺ)、齐墩果酸-3-O-β-D-葡萄糖醛酸甲酯苷(ⅩⅡ)。结论化合物Ⅱ、Ⅲ、Ⅸ、Ⅹ、ⅩⅡ为首次从本属植物中分离得到,化合物Ⅰ、Ⅳ、Ⅴ为首次从该植物中分离得到。
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[Abstract]
Objective For obtaining a more comprehensive understanding on the effective components,chemical constituents from the roots of Pfaffia paniculata were studied.Methods The chemical consti-tuents were isolated and purified by silica gel and Sephadex LH-20 column chromatography.Their structures were elucidated by chemical and spectral analyses.Results Twelve compounds were obtained and identified as ecdysterone(Ⅰ),3-methoxy-4-hydroxy-benzoic acid(Ⅱ),oleanolic acid 3-O-β-D-glucurono-pyranoside(Ⅲ),oleanoic acid 28-O-β-D-glucopyranoside ester(Ⅳ),oleanolic acid(Ⅴ),stigmasterol-β-D-glucoside(Ⅵ),stigmasterol(Ⅶ),daucosterol(Ⅷ),β-sitosterol(Ⅸ),20-hydroxyecdysone-20, 22-monoacetonide(Ⅹ),1-O-β-D-glucopyranosyl-(2S,3S,4R)-2-[2′(R)-hydroxyl hexadecosanoyl-amino]-4-octadecene-1,3-diol(Ⅺ),and oleanolic acid 3-O-β-D-glucuronopyranoside-methyl ester(ⅩⅡ).Conclusion Compounds Ⅱ,Ⅲ,Ⅸ,Ⅹ,and ⅩⅡ are isolated from this genus for the first time and compounds Ⅰ,Ⅳ,and Ⅴ are isolated from this plant for the first time.
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