目的研究滇重楼皂苷成分滇重楼总皂苷、滇重楼皂苷Ⅰ、薯蓣皂苷元的抗肺癌活性。方法以小鼠肺腺癌细胞株LA795及其宿主近交系T739荷瘤小鼠为研究模型,采用MTT法检测滇重楼总皂苷、滇重楼皂苷Ⅰ和薯蓣皂苷元的细胞毒活性,荷瘤小鼠实验检测抑瘤率、组织病理形态变化及瘤细胞转移情况。结果MTT结果显示滇重楼总皂苷、滇重楼皂苷Ⅰ和薯蓣皂苷元对LA795细胞生长有很强的抑制作用,其IC50分别为24.33μg/mL,1.85、149.75μmol/L;
各受试药物高剂量组对T739荷瘤小鼠抑瘤率分别为41.82%、29.44%、33.94%;
滇重楼总皂苷给药组可不同程度抑制肿瘤细胞的肺转移和肝转移。结论滇重楼总皂苷成分在体外、体内皆有强的抗肺癌作用。

Objective To study the antitumor activity of steroid saponins,such as Rhizoma Paridis saponins,Rhizoma Paridis saponin Ⅰ,and diosgenin,extracted from Paris polyphylla var.yunnanensis against lung adenocarcinoma cells.Methods Taking mouse lung adenocarcinoma LA795 cell line and its T739-bearing mice as models,the antitumor activity of steroid saponins of P.polyphylla var.yunnanensis against lung adenocarcinoma cells was carried out in vitro and in vivo.The inhibitory activity of Rhizoma Paridis saponins,Rhizoma Paridis saponin Ⅰ,and diosgenin on the LA795 cell line was studied by means of MTT assay and their antitumor effects in vivo were carried on inbred strains of mice(T739) affected by LA795 metastatic lung cancer.Results The MTT assay of in vitro cytotoxicity of Rhizoma Paridis saponins,Rhizoma Paridis saponin Ⅰ,and diosgenin showed strong cytotoxicity against the growth of LA795 cells.IC50 Values were 24.33 μg/mL,1.85,and 149.75 μmol/L,respectively.The tumor weight in all treated groups was significantly lower than that in the control group and the inhibition of tumor growth were 41.82%,29.44%,and 33.94% in their high-dose groups.The metastasis of tumor cells was inhibited by different degrees in treated groups.Conclusion Steroid saponins of P.polyphylla var.yunnanensis have tumoricidal activity on lung adenocarcinoma cell line,both in vitro and in vivo.

天津市自然科学基金重点项目(07JCZDJC05400)