目的揭示党参根的发生发育规律及储藏物质和主要药用成分在根中的积累部位。方法应用石蜡切片法研究党参根的形态发生,PAS反应和苏丹Ⅲ染色显示根中储藏物质,等量的5%香草醛-冰醋酸和高氯酸混合试剂对党参萜类及甾类物质显色。结果党参根发育过程包括原分生组织、初生分生组织、初生结构和次生结构4个阶段。党参根中存在大量乳汁管,在原生木质部导管出现时,韧皮部中已产生乳汁管细胞,而后一直与韧皮部分子相伴产生。党参根中积累了大量淀粉粒。萜类和甾类物质积累在根的薄壁细胞和乳汁管中。结论党参根中大量薄壁细胞和乳汁管是储藏物质和主要药用成分的积累部位。

Objective To study the process of the root development of Codonopsis pilosula as well as theaccumulatinglocationsofthemainmedicinalcompositionandstoragenutritionintheroot.MethodsThe development process of the roots was studied by paraffin section methods. Storage nutrition was loca-lized by PAS reaction and Sudan Ⅲ reaction. The medicinal composition of C. pilosula, terpene and steroid were stained with mix reagent of 5% vanillin-acetic acid and perchloric acid. Results The development process of the root is shown as four stages: promeristem, primary meristem, primary structure, and secondary growth. Abundant laticifers exist in mature root and the prime laticifers could be found in phloem as early as the first vessel of primary xylem appears. Then, the laticifers exist in phloem at all time. Starch granule accumulated in mature root as reserved substance. Terpene and steroid accumulated in parenchyma and laticifer. Conclusion Parenchyma and laticifer in the roots of C. pilosula are the main parts of the roots to store medicinal composition and storage nutrition.

陕西省教育厅专项基金(06JK80)