目的研究补骨脂素(PSO)加长波紫外线(UVA)光化学疗法(PUVA)诱导人白血病细胞K562、NB4凋亡时对线粒体膜电位的影响。方法细胞与不同质量浓度PSO,接受或不接受UVA照射后共同培养,电镜下观察细胞超微结构改变,流式细胞仪检测细胞凋亡率和线粒体膜电位变化,采用多因素方差分析法进行统计学处理。结果PUVA处理后的K562、NB4细胞超微结构出现明显的凋亡形态学改变;PSO、UVA照射及PUVA均可使细胞凋亡率增加,线粒体膜电位下降,PUVA的作用显著强于前两者(P<0.0)。结论PUVA可诱导人白血病细胞发生凋亡,诱导凋亡的途径之一为下调线粒体膜电位水平。

Objective To study the effects of psoralen (PSO) and long wave ultraviolet A (UVA) [PSO+UVA (PUVA)] on mitochondrial membrane potential in apoptosis of K562 and NB4 leukemia cells. Methods Cells were incubated with PSO in different concentration irradiated with or without UVA. The changes of ultrastructure of the cells were observed under the electron microscope. The apoptosis rates and the changes of mitochondrial membrane potential were detected through the flow cytometry. The factorial design and analysis of variane were used to analyze the interaction among the factors. Results There were obvious ultrastructure changes in apoptosis of K562 and NB4 leukemia cells after treated with PUVA. PSO, UVA, and PUVA all increased the apoptosis rates and decreased the mitochondrial membrane potential, and the effects of PUVA were stronger than that of the other two (P0.01). ConclusionPUVA could induce the apoptosis of K562 and NB4 leukemia cells and one of the pathway to induce apoptosis is to downregulate the mitochondrial membrane potential.

国家自然科学基金资助项目(30472257)