目的 观察复肾降纤宁对糖尿病肾病(DN)大鼠肾脏蛋白激酶C(PKC)、血管内皮生长因子(VEGF)和血小板衍生生长因子-β(PDGF-β)的影响及对肾脏的保护作用。方法 建立链脲佐菌素(STZ)诱导的糖尿病肾病(DN)大鼠模型,将成模糖尿病大鼠随机分成4组:模型组、复肾降纤宁组、厄贝沙坦组、厄贝沙坦+复肾降纤宁组,同时取正常大鼠设对照组。各组大鼠采用相应的干预措施处理12周。常规方法 检测各组大鼠肾脏指数、血糖、尿素氮(BUN)、血肌酐(Scr)、尿白蛋白排泄率(UAER),免疫组化法检测肾PKC和VEGF表达,Western blotting检测肾组织PDGF-β的表达,透射电镜观察肾脏超微结构。结果 模型组大鼠肾皮质PKC、VEGF和PDGF-β表达显著升高,肾脏超微结构改变明显,血糖、UAER、BUN、Scr、肾脏指数显著增高;复肾降纤宁组、厄贝沙坦组和厄贝沙坦+复肾降纤宁组上述指标显著低于模型组(P<0.05),肾脏超微结构改变明显改善;厄贝沙坦+复肾降纤宁组各项指标改善明显优于模型组、复肾降纤宁组和厄贝沙坦组(P<0.05),但未达到对照组水平。结论 复肾降纤宁对DN大鼠肾脏形态和功能有明显保护作用,其机制可能与减少肾组织中PKC、VEGF和PDGF-β的表达,减轻肾组织纤维化有关。

Objective ?To?investigatethe?effeet?ofFushenjiangxianningon?renal?protein kinase C?(PKC),renal vascular endothelialgrowthfactor(VEGF),renal platelet-derivedgrowthfactor p(PDGF-?13),and renal protection in diabetic nephropathy(DN)rats.Methods ?The diabetic SD rats induced by?STZ were divided into fourgroups:diabetic eontrol group,Fushenjiangxianning group,Irbesartan group,andFushenjiagxianningcombined with Irbesartangroup,andthe normal controlgroupas well. After the?experimental rats in everygroupwere respectively treated for 1 2 weeks by above interventionMethods ,the?relative kidney weight,blood sugar,urea nitrogen(Bun),serum creatinine(Scr),and urinary albumin?excretion rate(UAER)were detectedbyroutineanalysis Methods ,and PKC and VEGF in renal tissue?were detectedbyimmunohistochemicaltechniques,and PDGF-pin renal?tissue was detectedbyWestern?blotting,anduhramicrostructure of kidney was observed by transmission electron microscope.Results ?Theexpression of renal cortex PKC and VEGF was increased in diabetic rats.The uhramicrostructureoC?kidneywasnoticeably changed,andthe bloodsugar,UAER,BUN,Scr,relative kidney weight,and?PDGF-p were?significantlyincreased?in?diabetic?rats.?While?the above indexes?in Fushenjiangxianning?group,?Irbesartan?group, and Fushenjiangxianning combined with Irbesartan groupwere?significantly?lower than those in diabetic controlgroup(P<0.05),theuhramicrostructure ofkidney changedwas?improved,and the improvement of all indexes in Fushenjiangxianningcombined with Irbesartangroupwas?the most obvious(P<0.05).Conclusion ?The mechanism of the protection of Fushenjiangxianning on?renal lesionmaybedecreasingthe level ofPDGF-pin the renal tissue anddownregulatingtheexpressionof?PKC and VEGF.

重庆市卫生局资助项目(渝中医[2003]32号B-26)