目的 利用高效毛细管电泳技术建立空心莲子草药材的指纹图谱。方法 采用60cm×75μm毛细管柱,以50mmol/L硼砂溶液-50mmol/L磷酸盐溶液为缓冲液,运行电压为20kV,检测波长220nm,柱温25℃。结果 建立的指纹图谱中共有18个共有峰,且方法学考察符合规定的标准。结论 该法准确简便,可作为控制空心莲子草药材内在质量的有效手段。

Objective To investigate the high performance capillary electrophoresis(HPCE) fingerprint of Alternanthera philoxerodes. Methods Separation was performed on a 60 cm×75 μm uncoated capillary with 50 mmol/L borate solution-50 mmol/L phosphate solution (pH 8.76) as HPCE buffer.The running voltage was 20 kV,the detection wavelength was 220 nm and temperature 25 ℃. Results Fingerprint was established with 18 common peaks. Conclusion The method is accurate,simple,and suitable to the quality control of A. philoxerodes.

国家自然科学基金资助项目(30470194)