[关键词]
[摘要]
目的 建立叶下珠愈伤组织培养方法。方法 采用组织培养方法,比较不同外植体、蔗糖、植物生长物质及其配比对叶下珠愈伤组织诱导的影响。结果 茎段的诱导率最高,为55.56%,叶片诱导不出愈伤组织。6-BA是叶下珠愈伤组织形成的主要影响因素,2,4-D、NAA、蔗糖次之。结论 叶下珠愈伤组织诱导及继代的适宜培养基为MS+2,4-D0.5mg/L+6-BA1.0mg/L+NAA0.1mg/L+蔗糖10g/L。
[Key word]
[Abstract]
Objective To establish culture method for Phyllanthus urinaria. Methods To study the possible effective factors of culture condition by comparing with different explants,sucrose,plant growth substance,and its ratio. Results The inductivity of stem was the highest about 55.56%,but callus of leaves could not be induced.6-BA was the most important factor on callus induction,followed by 2,4-D NAA and sucrose. Conclusion The optimal medium to induce and subculture is MS+2,4-D 0.5 mg/L+6-BA 1.0 mg/L+NAA 0.1 mg/L+sucrose 10 g/L.
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[基金项目]
国家农业成果转化基金项目(05EFN213400124);淮北市重大专项(06085)