[关键词]
[摘要]
目的 以杭白菊子叶和下胚轴为外植体进行组织培养,寻找适宜诱导菊花子叶和下胚轴愈伤组织发生和分化的激素配比。方法 采用不同的激素配比MS培养基对杭白菊子叶和下胚轴分别培养。结果 试验设计的培养基均能诱导产生愈伤组织,但不同的激素配比对芽诱导分化的影响不同。其中MS+IAA0.3mg/L+6-BA12mg/L诱导菊花子叶和下胚轴得到的愈伤组织粗大且致密;MS+IAA0.3mg/L+6-BA4mg/L适宜子叶不定芽的诱导,诱导率为67.5%;MS+KT2mg/L+NAA0.2mg/L适宜下胚轴不定芽的诱导,诱导率为62.5%;生根培养采用1/2MS+NAA0.1mg/L,7d后生根率为100%。结论 本实验建立了以杭白菊子叶和下胚轴为外植体的高频快繁体系,不定芽诱导频率高且生长健壮。
[Key word]
[Abstract]
Objective To seek the optimization hormone combination for the callus induction and differentiation from cotyledon and hypocotyl explants of Chrysanthemum morifolium through tissue culture.Methods Cotyledon and hypocotyl explants were cultured on various medium with different hormone combinations.Results Callus could be inducted on every mediadesigned in this experiment,but the effects to the induction and differentiation culture of buds were different.The results showed that the medium MS+IAA O.3mg/L+6一BA 12mg/Lwas suitable to the induction of callus from cotyledon and hypocotyl.The optimal hormone combination was MS+IAA 0.3 mg/L+6一BA 4 mg/Lfor the initiation of adventitious bud ofcotyledon.Theshootregeneration percentagereached 67.5%.Theoptimalmedium for the initiation of adventitious bud of hypocotylwas MS+KT 2 mg/L+NAA 0.2 mg/L.The shoot regeneration percentage reached 62.5%.Rootingwas induced on 1/2 MS+NAA 0.1 mg/L,andthe root inductingratio reached 100%in 7d.Conclusion A rapid plantlet regeneration systemfor C.morifolium is established from cotyledon and hypocotyl explants.Bud induction frequency ishigherand the shoots in vitrogrow vigorously.
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[基金项目]
江苏省科技厅高技术研究项目(BC2005318)