目的 探讨大黄素对人高转移卵巢癌抗肿瘤的可能作用机制。方法 应用肿瘤基因芯片检测大黄素(40μmol/L)对人高转移卵巢癌HO-8910PM细胞中癌相关基因表达的影响,并且实时定量PCR和Western blotting进行验证。结果 共筛选出表达差异基因69个,其中33个基因表达水平上调,36个基因表达水平下调,涉及细胞凋亡、细胞周期、细胞生长与分化、细胞运动、信号转导、基因转录和细胞代谢等7大类相关基因。结论 基因芯片结果提示大黄素抗肿瘤作用可能与转化生长因子-β(TGF-β)信号转导通路密切相关。

Objective To explore the mechanism of antitumor effect of emodin on highly metastatic ovarian carcinoma HO-8910PM ceils.Methods The cancer-related gene expression profiles of HO-8910PM cells with and without emodin-treatment were analyzed using genechip technology.To validate the gene chip data，some differential expression genes，including BRCAl，GDFl5，and NRIDl were analyzed by fluorescent quantitative real-time PCR and Western hlotting assay.Results Sixty-nine differently expressed genes were screened out of which up-and down-regulated geneswere 33 and 36，respectively.These geneswere related to apoptosis，cell cycle，cell growthand differentiation，cell motility，signal transduction，transcription，and metabolism.Conclusion The results indicats that the antitumor effect ofemodin on HO-8910PM cells is involved in TGF-beta signaling pathway.

粤港科技合作项目(GHP/022/06);广东省中医药局课题(1050047);广东省科技计划项目(2007B030702003)