目的 观察左归复方(滋阴益气活血解毒组方)干预治疗MKR小鼠后糖代谢及抗氧化应激的影响。方法 50只MKR小鼠经鉴定后,随机分为MKR模型组,左归复方高、中、低剂量组,阳性对照组(文迪雅),每组10只。各药物组分别以相应剂量连续ig给药30d。于给药前、给药第15天及第30天,分别测定空腹血糖(FBG)。末次给药后0.5h,心脏取血,放射免疫法测血清胰岛素,比色法测定心、肾和肝组织中总抗氧化能力(T-AOC)、丙二醛(MDA)水平、超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-Px)的活性。结果 左归复方干预治疗后,其高剂量具有显著的降低MKR小鼠空腹血糖、改善高胰岛素血症的作用(P<0.05、0.01),且呈剂量依赖性;MKR小鼠心、肾和肝组织中MDA水平显著高于C57野生鼠组(P<0.05、0.01),而T-AOC及SOD和GSH-Px活性显著低于C57野生鼠组(P<0.01)。左归复方干预治疗后,能显著降低MKR小鼠心、肝和肾组织中MDA水平(P<0.05、0.01),提高各组织T-AOC,显著增强SOD和GSH-Px的活性(P<0.05、0.01),其作用呈剂量依赖性。结论 左归复方具有显著地改善MKR小鼠糖代谢,保护心、肝和肾的抗氧化酶,抑制氧化应激反应,对MKR小鼠产生保护作用。

Objective To observe the effect of Zuogui Recipe on glucose metabolism and antioxidative stress in MKR mice. Methods Fifty MKR mice were identified and randomly grouped. They were model group (MG),low Zougui Recipe group (LZG),middle Zuogui Recipe group (MZG),high Zuogui Recipe group (HZG),and positive control group (Rosiglitazone Maleate Tablets,PCG),ten mice each group. LZG,MZG,HZG,and PCG were treated by ig corresponding drug for 30 d. Using C57 mice as controls,the fasting blood glucose (FBG) level was determined before administration,after administration day 15,and day 30,respectively. And the serum insulin level was determined by radioimmunoassay. Total antioxidative capacity (T-AOC),malondiadehyde (MDA) levels,superoxide diamutase (SOD),and glutathioneperoxidase (GSH-Px) activities in heart,kidney,and liver tissue were determined by spectrophotometric method in 0.5 h of last administration. Results After treating MKR mice with Zuogui Recipe,the hyperglycemia was significantly ameliorated and the serum insulin in HZG was decreased (P0.05 and 0.01) in a dose dependent manner. The levels of MDA were significantly higher (P0.05 and 0.01),while capacity of T-AOC and activities of SOD and GSH-Px were significantly lower (P0.01) in heart,kidney,and liver tissue of MKR mice than those of C57 mice. After treating with Zuogui Recipe,the levels of MDA were significantly decreased (P0.05 and 0.01) and capacity of T-AOC,activities of SOD and GSH-Px in heart,kidney,liver tissue of MKR mice was significantly increased (P0.05 and 0.01) in a dose dependent manner. Conclusion The mechanism of protective effect of Zuogui Recipe on heart,kidney,and liver may be partly correlated with increasing the antioxidative capacity in MKR mice.

国家自然科学基金资助项目(30400604);国家中医药管理局回国留学人员项目(国中医药发[2004]68号-1);湖南省教育厅重点项目(04A040);湖南省自然科学基金杰出青年基金项目(08JJ1005)