目的 从银杏叶中分离纯化白果内酯和银杏内酯A、B、C。方法 银杏叶提取液经醋酸乙酯萃取、D-101大孔吸附树脂柱和Al₂O₃柱纯化后得到总内酯提取物,提取物再经两次高速逆流色谱分离制备4种内酯单体。结果 25%乙醇热提取、醋酸乙酯萃取、D-101柱、pH4.0的Al₂O₃柱对总内酯的提纯最终使总内酯质量分数达到44.98%。经高速逆流色谱制备得到的白果内酯和银杏内酯A、B、C最高质量分数分别为98.3%、98.9%、98.8%、98.4%。结论 本法简便快速、回收率高,为银杏叶中内酯单体的制备提供了一种新方法。

Objective To separate and purify bilobalide and ginkgolide A，B，and C from Ginkgo biloba leaves.Methods Firstlythe extract of G. biloba leaves was purified by extraction of acetic ether，D一101 macroporous resin，and Al₂O₃ (pH4) to obtain the crude extract of lactones.Then the crude extract was further purified by high-speed counter-current chromatography (HSCCC) to prepare the monomer of bilobalide andginkgolide A，B，and C.Results The crude extract of lactones with thepurity of 44.98% was obtained by extraction with 25% alcohol，acetic ether，D-101 macroporous resin，and Al₂O₃ (pH4).When the crude extract was further purified by HSCCC，the different purity of bilobalide and ginkgolide A，B，and C wasgot.Themaximalpurityof bilobalide andginkgolide A，B，and C was 98.3%，98.9%，98.8%，and 98.4%.Conclusion This method，which is simple and rapid，provides a new way to separate andpurify the lactones from G.biloba leaves.

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