[关键词]
[摘要]
目的揭示栽培黄连Coptis chinensis群体的遗传关系。方法以24个不同来源地的栽培黄连群体为试材,采用SRAP分子标记技术,用Treeconw软件分析遗传相似系数,UPGMA方法聚类,构建遗传系统树。结果36对引物共得到276条扩增条带,其中有120条呈现多态性,占43.48%,从DNA分子水平显示出供试种质遗传多样性并不丰富。遗传相似系数变化范围在0.877 0~0.951 9。聚类结果显示栽培黄连群体遗传关系与来源地无明显相关性,仅在小分支中表现出一定的地域相关性。结论栽培黄连群体的遗传多样性水平较低,显示遗传背景较为单一。
[Key word]
[Abstract]
Objective To reveal the genetic relationship among populations of cultivated Coptis chinensis.Methods Twenty four populations of cultivated C.chinensis from different habitats wereemployed to be analyzed by the approach of sequence-related amplified polymorphism(SRAP).Systematicrelationship was constructed based on the UPGMA method by Treeconw software.Results A total of 276bands were scored,among which 120 were polymorphic bands.The average percentage of polymorphicbands was 43.48%,indicating that the materials in the test have low genetic diversity.Genetic similaritycoefficients were changed from 0.877 0 to 0.951 9.By cluster analysis,the geographical distribution was not very obvious。but it was also showed some of the cultivated C.chinensis from the same region were inthe same group.Conclusion Different germplasms diversity of cultivated C.chinensis population is lowerand genetic background is more single.
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[基金项目]
国家科技攻关计划资助项目(2004BA721A32);国家中医药管理局攻关项目(国中医药科2004ZX06-1);重庆市科技计划资助项目(8847)