[关键词]
[摘要]
目的研究无机陶瓷微滤膜纯化虫草粗多糖溶液,确定操作工艺条件和有效清洗方法。方法主要考察膜通量和膜通量恢复率。结果在60℃,pH 7~8、0.3~0.4 MPa压差操作条件下微滤虫草粗多糖溶液,多糖收率较传统醇提工艺高出30%,同时可除去大部分不溶固体物和蛋白质胶体等大分子有机物杂质。采用80℃热水与1%NaOH溶液交替清洗并伴有间歇、短时反冲洗,可使膜的清水通量恢复率达到90%以上。结论无机陶瓷微滤膜纯化虫草粗多糖溶液工艺简单、可行。
[Key word]
[Abstract]
Objective To apply the membrane technology for purifying rough cordyceps polysaccha- rides solution,the optimal operation condition and effective cleaning method were researched.Methods To mainly analyze the flux of membrane and its recovery.Results The optimal experiment operation condition obtained is 60℃,pH value 7-8,and pressure 0.3-0.4 MPa,by which the polysaccharides recovery was about 30%,more than by traditional ethanol extraction,and the most impurities as infusibility substance and protein colloid etc.were taken out.Moreover,the flux of membrane recovery can be above 90%after 80 oC water and 1%NaOH solution was alternately used to wash the fouling membrane under in- termittent little back-pulsing.Conclusion The process of membrane technology purifying rough cordyceps polysaccharides is simpl and feasible.
[中图分类号]
[基金项目]
北京市教委项目(KM200511417005)