[关键词]
[摘要]
目的: 为了改进TTC法测定肉苁蓉种子活力的方法和调查肉苁蓉种子于5℃贮藏时种子活力的变化。方法: 研究了TTC法中种皮、TTC溶液、次氯酸钠(NaClO)溶液和染色时间对肉苁蓉种子活力的影响, 并用改进的方法测定了于5℃贮藏了不同年限种子活力。结果: 肉苁蓉种子于40℃下用0.5%TTC溶液染色48h后, 再用0.2% NaClO溶液漂白2h后镜检; 采收后贮藏于5℃保存1~2年的种子,其活力没有明显变化, 但高活力种子的百分比随着贮藏年限的增加而增加。结论: 提供了一套方便快捷的测定肉苁蓉种子活力的方法; 肉苁蓉种子于5℃贮藏有利于提高种子活力。
[Key word]
[Abstract]
Objective: To improve the 2, 3, 5-triphenyl tetrazolium chloride(TTC)solution method for measuring the viability of Cistanche deserticola seeds and investigate the change in viability during storage at 5℃.Methods: The effect of the testa, TTC concentration,sodium hypochlorite concentration (NaClO), and staining time were studied, and seed viability during storage at 5℃ was measured with the improved method.Results: Seeds were kept for 48 h in 0.5% TTC solution at 40℃, and then for 2h in0.2% NaCl0 solution; Seed viability was measured under a stereomicroscope. Storing seeds of C.deserticola for 1 to 2 years at 5℃had no significant effects on their viability. However, the percentage of seeds with high viability was increased with the extension of the storage time at 5℃.Conclusion: A convenient and rapid method for measuring the viability of C.deserticola seeds is developed. Storing C. deserticola seeds at 5℃ could improve their viability.
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[基金项目]
河北省科技攻关项目(03276408D-4);重大基础研究前期研究专项(2004CCA01200)