[关键词]
[摘要]
目的: 研究草珊瑚的遗传多样性及不同种源之间的遗传关系, 为保护种质资源奠定基础。方法: 采用ISSR分子标记对8个种源共51个草珊瑚样品进行了DNA分子水平的分析评价, 用Popgen32软件计算遗传多样性和遗传距离, 通过UPCMA法进行聚类分析。结果: 筛选出10条多态性ISSR引物用于草珊瑚不同样品的ISSR-PCR, 检测得到111个位点, 其中106个为多态性位点, 约占95.50%。草珊瑚不同种源间遗传距离在0.0347~0.1850。8个种源总遗传多样性主要来自种源内遗传变异, 种源间分化指 Gst=0.3403。结论: 8个草珊瑚种源大体可以分成2大类群。且根据遗传距离与产地海拔高度的关系, 可以将其划分为较高海拔类群(800~900m)、较低海拔类群(250~300m)、中等海拔类群(600m)。
[Key word]
[Abstract]
Objective: Sarcandra glabra is a widely-used Chinese medicinal herb, but the wild germplasm resources were decreasing. In order to protect the germplasm resources, the genetic diversity and genetic relationship among the various provenances should be analyzed. Methods: ISSR Molecular markers were used to analyze the genetic diversity of S.glabra collected from eight provenances. The genetic diversity, genetic distance, and cluster analysis were performed by Popgen32 software and UPCMA method. Results: Ten screened polymorphic primers were used in ISSR-PCR and 111 bands were amplified, among them including 106 polymorphie bands that were about 95.50%. Genetic distance of eight different provenances were ranged from 0.0347-O.1850. The genetic diversity of S.glabra mainly came from the interior variation of every provenance, for Gst=0.3403. Conclusion: Provenances of S.glabra can be divided into two groups and based on the relationship of genetic distance and altitude, the provenances can be divieded as follows:correspondin91y higher altitude group(800-900 m); correspondingly lower altitude group(250-300 m); middling altitude group(60m).
[中图分类号]
[基金项目]
浙江省科技计划项目(2006C23008)