[关键词]
[摘要]
目的了解川贝母种子休眠原因及层积处理打破种子休眠的作用。方法浸种称重法测定种皮透水性;生物鉴定法检测发芽抑制物;解剖种子观察层积种子种胚发育;光照培养箱发芽检测层积种子萌发温度。结果浸种8h后川贝母种子吸水率达80%以上;种子浸提液显著抑制白菜种子的萌发和生长;层积处理促进种胚后熟,60d后胚率由处理前12%增至43%;层积种子25℃发芽率显著高于其他发芽温度。结论川贝母种皮无吸水障碍;种子休眠的主要原因是胚形态后熟及存在发芽抑制物,属于混合休眠类型;层积处理可有效打破休眠,25℃发芽适温。
[Key word]
[Abstract]
Objective To investigate the mechanism of the seed dormancy of Fritillaria cirrhosa and the stratification effect on seed dormancy. Methods The soaked seeds were used to test water permeability by weighting. The biological test was applied for detecting the germination inhibitor. Seed dissection was used to investigate the embryo development after stratification. The temperatures of seeds germination after stratification were determined by artificial climate chamber at different temperatures.Results The water absorption rate of F. cirrhosa seeds was more than 80% after soaking for 8 h. The extracts significantly inhibited both the germination of seed and the root growth of Chinese cabbage. The stratificaton could effectively accelerate the embryo afterripening and increase the embryo rate from 12% to 43% after 60 d. Germination rate of the seed after stratificaton in 25 ℃ was significantly higher than that in other temperatures. Clousion There is no obstacle of water permeability on testa of F. cirrhosa. The seed embryo afterripening and the germination inhibitor are the main mechanism of seed dormancy of F. cirrhosa, whereas it is a type of mix-dormancy. The stratification is effective for seed dormancy relief and the optimum germination temperature is 25 ℃.
[中图分类号]
[基金项目]
国家高技术产业化专项(2005-1);北京市科技新星计划项目(2004A60);国家中医药管理局科技专项(2004ZX06-2);国家中药材生产扶持资金项目(2005-1)