目的观察荜茇宁对实验性高脂血症大鼠血脂代谢及其相关基因表达的影响。方法饲喂高脂饲料建立高脂血症大鼠模型,荜茇宁分别按2.5、5、10mg/kg给大鼠连续ig4周。实验结束,取空腹血,检测血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、载脂蛋白A1(ApoA1)、载脂蛋白B(ApoB);利用反转录聚合酶链反应(RT-PCR)测定大鼠肝脏低密度脂蛋白受体(LDLR)、ApoB和3-羟基-3-甲基-戊二酰辅酶A还原酶(HMG-CoAR)mRNA的表达。结果与模型组相比,荜茇宁能降低血清TC、TG、LDL-C、ApoB水平及ApoB/ApoA1的值(P<0.05),升高HDL-C、ApoA1(P<0.05),能增强高脂血症大鼠LDLR mRNA表达(P<0.05),降低ApoB mRNA表达(P<0.05),但对HMG-CoAR mRNA的影响不明显(P>0.05)。结论荜茇宁具有调节高脂血症大鼠血脂代谢的作用,其机制可能与提高LDLR基因转录水平,降低ApoB mRNA表达有关。

Objective To observe the effects of piperlonguminine on the blood lipid metabolism and its related genes expression in hyperlipidemia rats. Methods The hyperlipidemia models induced by high-fat feeding were established. The piperlonguminine group was fed continuously with piperlonguminine (2.5, 5, and 10 mg/kg) for four weeks. At the last day, all the rats fasted for at least 12 h and the blood was drawn for the measurement of total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), apolipoproteinA1 (ApoA1), and apolipoprotein B (ApoB); The mRNA expression of low density lipoprotein cholesterol receptor (LDLR), ApoB, and 3-hydroxy 3-methylglutaryl co-enzyme A reductase (HMG-CoAR) were measured by RT-PCR. Results Compared with that of the high-fat model group, the serum TC, TG, LDL-C, ApoB levels, and the ratio of ApoB/ApoA1 were reduced (P0.05) and the serum HDL-C and ApoA1 were increased in the piperlonguminine group (P0.05). Piperlonguminine could increase hepatic LDLR mRNA levels (P0.05) and reduce ApoB mRNA levels (P0.05). But it had no significant effects on HMG-CoAR mRNA level (P0.05).Conclusion Piperlonguminine has the effect of regulating lipid metabolism. The mechanism is likely related to increasing LDLR mRNA expression and decreasing ApoB mRNA expression.

国家自然科学基金资助项目(30160103)