目的探讨通腑醒神胶囊对大鼠急性局灶性脑缺血再灌注损伤的神经保护作用是否与调节神经细胞膜上Na离子通道基因表达有关。方法观察通腑醒神胶囊治疗对大脑中动脉阻塞模型大鼠各时间点神经功能缺损评分、脑含水量、脑梗死体积的影响;运用荧光定量RT-PCR技术检测各组动物损伤侧及健侧大脑神经细胞膜上Na离子通道α亚基基因各亚型(Nav1.1、Nav1.2、Nav1.3、Nav1.7、Nav1.8)mRNA在不同时间点的表达水平。结果治疗组在1、2、3、7d时神经功能缺损评分,3d时脑含水量,3、7d时的脑梗死体积均较模型组低,差异显著(P<0.01);治疗组损伤侧脑组织在1、2d时Nav1.1mRNA表达水平高于模型组,其中2d时差异显著(P<0.05),但低于健侧、假手术组及对照组(P<0.05);模型组及治疗组损伤侧Nav1.2、Nav1.3、Nav1.7、Nav1.8mRNA的表达水平与健侧、假手术组及对照组相比,在各时间点均未见显著性差异(P>0.05)。结论通腑醒神胶囊对缺血性神经细胞具有保护作用,可能与调节Nav1.1表达有关。

Objective To study the relationship between the neural protection of Tongfu Xingshen Capsula and the expression of sodium channel genes in rats with acute ischemic brain injury. Methods The neurologic impairment score, brain water content, and infarct volume were observed at different time points in the right middle cerebral artery occlusion (MCAO) rats. Using fluorescence quantitative RT-PCR technique to measure the expression of sodium channel genes (Nav1.1, Nav1.2, Nav1.3, Nav1.7, and Nav1.8) mRNA in both injured and contralateral hemispheres at different time points. Results At 1, 2, 3, and 7 d time-points, the neurologic impairment scores of rats in Tongfu Xingshen Capsula group were lower than those in model group with significant differences (P0.01). At 3 d time-point, the brain water content of rats in Tongfu Xingshen Capsula group was decreased significantly (P0.01) comparing with model group. At 3 and 7 d time-points, the infarct volume of rats in Tongfu Xingshen Capsula group was significantly lower than those in model group (P0.01). At 1 and 2 d time-points, the expression of Nav1.1 mRNA in injured hemisphere of rats in Tongfu Xingshen Capsula group was higher than that in model group especially significant at 2 d time point (P0.05), but was lower than that in contralateral side, Sham-operated group, and normal control group (P0.05). However, there were no signifcant differences among the expression of Nav1.2, Nav1.3, Nav1.7, and Nav1.8 mRNA in injured and contralateral hemispheres of all groups at all observed time-points (P0.05). Conclusion The findings suggest that the neural protection of Tongfu Xingshen Capsula on ischemic injury brain maybe contributes to regulate the expression of Nav1.1 sodium channel gene.

国家自然科学基金项目(30400583)