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[摘要]
目的研究轮叶婆婆纳Veronica sibirica中二萜类成分及其体外抗肿瘤作用。方法应用现代提取分离和波谱分析方法对轮叶婆婆纳进行提取、分离、纯化和结构鉴定;以SK-N-SH和BEL-7402肿瘤细胞株为研究对象,采用MTT法对化合物进行体外抗肿瘤活性研究。结果从轮叶婆婆纳中分离鉴定了一个新二萜类化合物,鉴定为1,2-去氢隐丹参酮(Ⅰ);其他7个已知化合物:轮叶婆婆纳对醌A(Ⅱ)、轮叶婆婆纳对醌B(Ⅲ)、隐丹参酮(Ⅳ)、弥罗松酚(Ⅴ)、二氢丹参酮Ⅰ(Ⅵ)、丹参酮Ⅰ(Ⅶ)、丹参酮ⅡA(Ⅷ)。体外抗肿瘤活性实验结果显示化合物Ⅱ,Ⅳ~Ⅷ对SK-N-SH肿瘤细胞生长的抑制作用较强,化合物Ⅱ,Ⅳ,Ⅵ,Ⅷ对BEL-7402肿瘤细胞生长的抑制作用较强。结论轮叶婆婆纳中的二萜类化合物对不同种肿瘤细胞具有细胞毒选择性,有一定的抗癌活性。
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[Abstract]
Objective To study the chemical constituents of the whole plant of Veronica sibirica and to examine the anti-tumor effect in vitro. Methods Various chromatographic techniques were used to separate and purify the constituents and their structures were elucidated on the basis of spectroscopic analysis. The inhibitory effects of these compounds on SK-N-SH and BEL-7402 cell lines were tested in vitro by MTT method. Results Eight compounds were isolated and identified as 1, 2-dehydrocryptotanshinone (Ⅰ), sibiriquinone A (Ⅱ), sibiriquinone B (Ⅲ), cryptotanshinone (Ⅳ), ferruginol (Ⅴ), dihydrotanshinone Ⅰ (Ⅵ), tanshinone Ⅰ (Ⅶ), and tanshinone ⅡA(Ⅷ). Compound Ⅰ is a new compound. Compounds Ⅱ, Ⅳ-Ⅷ show favorable inhibitory effects on the SK-N-SH cell growth, and compounds Ⅱ, Ⅳ, Ⅵ, Ⅷ show the inhibitory effects on the BEL-7402 cell growth. Conclusion The study suggests that the diterpenes from V. sibirica have the anti-tumor effects and these cytotoxic activities on different tumor cell lines are various and selective.
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[基金项目]
天津医科大学科研基金资助