目的
研究烟草悬浮培养细胞对呋喃橐吾酮(Ⅰ)的生物转化。
方法
将外源底物呋喃橐吾酮乙醇溶液投入预培养10d的烟草悬浮培养细胞中,共培养4d后终止转化,通过HPLC检测方法,并利用各种色谱技术分离纯化转化产物,最后根据其理化性质和光谱数据进行结构鉴定。此外,实验还考察了共培养时间对转化率的影响。
结果
呋喃橐吾酮(Ⅰ)在烟草细胞中发生了转化,分离鉴定出两个转化产物:3-oxo-eremophila-1,7(11)-dien-12,8-olide(Ⅱ)和3-oxo-8-hydroxyeremophila-1,7(11)-dien-12,8-olide(Ⅲ)。转化的最佳共培养时间为5d,此时总的摩尔转化率最高(53.1%)。
结论
首次利用烟草悬浮培养细胞转化倍半萜类化合物呋喃橐吾酮并获得成功。

Objective To investigate the biotransformation of furannoligularenone by cell suspension cultures of Nicotiana tabacum.Methods Furannoligularenone was added to the medium of the suspensioncells of N.tabacum after precuhured for 10 d.then they were co-cultured for another 4 d.Thebiotransformed products were detected with HPLC and isolated by various chromatographic methods.Thechemical structures of biotransformed products were elucidated on the basis of their physicochemical pro-perties and spectroscopic data.Otherwise,the influence of CO-cultured time on conversion ratio wasinvestigated either. Results The substrate,furannoligularenone(I),was successfully biotransformed by N.tabacum cultured cells.Two products,3-oxo-eremophila一1,7(11)-dien一12,8一olide(I)and 3-oxo-8一hydroxyeremophila一1,7(11)-dien一12,8-olide(I)were obtained.After CO-cultured for 5 d,the moleconversion ratio of the substrate reached the highest(53.1％).Conclusion It’S the first report 011biotransformation of furannoligularenone by N.tabacum cultured cells.