目的
探讨培养方式、碳源、氮源对山药微型块茎诱导形成的影响,以期找到适宜的微型块茎诱导形成的培养方式和培养基。
方法
以B号山药试管苗为材料,运用一步培养法和两步培养法筛选最佳培养方式,在此基础上于培养基中添加不同氮源和碳源进行诱导培养,定期对微型块茎的诱导情况进行统计。
结果
两步培养法、60g/L的碳源(白砂糖或蔗糖)、[NO3-]/[NH4+]为3:1、总氮量为30mmol/L时有利于山药微型块茎的诱导形成。
结论
首次建立了两步培养法诱导山药微型块茎形成的培养模式,筛选出了适宜的碳源和氮源,为微型块茎的离体诱导形成及工厂化生产奠定了技术基础。

Objective To assess the effect of culture method,carbon source,and nitrogen source on the induction of D.opposita microtubers and optimize the culture method and medium.Methods D.opposita CV.No.B was used as the materials。one-step and tWO-step cultivation were compared to optimizethe better culture method.On the base of different carbon sources and nitrogen sources added in the medium,the microtubers induced were evaluated regularly.Results Two-step cultivation,carbon sourcewith 60 g／L concentration(white sugar or sucrose),nitrogen source with 30 mmol／L total nitrogencontent,and the ratio of _N03^{一／NH₄+(3:1)were adaptable to the induction of D.opposita microtubers.Conclusion The induction mode of D.opposita microtubers with two-step cultivation has been established.the optimal carbon source and nitrogen source are obtained and a foundation iS laid formicrotubers induction in vitro and large scale production.}

国家自然科学基金资助项目(30670208);河南省重点科技攻关项目(0623030700);河南师范大学青年科学基金资助项目(2006036)