[关键词]
[摘要]
目的研究白头翁Pulsatilla chinensis地上部分的化学成分。方法应用硅胶和ODS色谱柱进行分离纯化,应用各种波谱技术(UV、IR、MS、NMR)鉴定化合物的结构。结果从白头翁地上部分的95%乙醇提取物中分离得到9个化合物,分别鉴定为L-菊苣酸(Ⅰ)、银椴苷(Ⅱ)、芹菜素-7-O-β-D-(3″-反式对羟基肉桂酰氧基)葡萄糖苷(Ⅲ)、4,6,7-三甲氧基-5-甲基香豆素(Ⅳ)、4,7-二甲氧基-5-甲基香豆素(Ⅴ)、myo-肌醇(Ⅵ)、莽草酸(Ⅶ)、1,4-丁二酸(Ⅷ)、5-羟基-4-氧代戊酸(Ⅸ )。结论所有化合物均为首次从该植物中分离得到,其中化合物为新的天然产物。
[Key word]
[Abstract]
Objective To study the chemical constituents in the aerial parts of Pulsatilla chinensis.Methods The compounds were isolated and purified by silica gel and ODS column chromatographies.Their structures were identified by physical and chemical properties as well as spectral data.Results Nine compounds were isolated from the 95% ethanol extract in the aerial parts of this plant.Their structures were elucidated as L-chicoric acid(Ⅰ),tiliroside(Ⅱ), apigenin 7-O-(3″-p-coumaryl)-glucoside(Ⅲ),4,6,7-trimethoxy-5-methyl coumarin(Ⅳ),4,7-dimethoxy-5-methyl coumarin(Ⅴ),myo-inositol(Ⅵ),shikimic acid(Ⅶ),1,4-amber acid(Ⅷ),and 5-hydroxy-4-oxo-pentanoic acid(Ⅸ),respectively.Conclusion Compound Ⅰ is a new natural product.All compounds are isolated from this plant for the first time.
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[基金项目]
国家杰出青年科学基金(30625039);广东省关键领域重点突破项目(2003A30909);广州市科技攻关计划(2004Z2-E5021)