[关键词]
[摘要]
目的研究香鳞毛蕨中的化学成分及其生物活性。方法利用柱色谱和高效液相色谱等现代技术对香鳞毛蕨的化学成分进行分离,根据理化性质和现代波谱技术并辅以化学方法对化合物进行结构鉴定,采用MTT法测定化合物对Bel-7402、HCT-8及A-549的细胞毒活性。结果共鉴定出4个化合物,分别为京尼平苷(geniposide,Ⅰ)、3S,5R,6R,7E,9S-四甲基环己烯型单萜-7-烯-3,5,6-9-四羟基-3-O-β-D-葡萄吡喃糖苷(3S,5R,6R,7E,9S-megastigman-7-ene-3,5,6,9-tetrol-3-O-β-D-glucopyranoside,Ⅱ)、(6S,9R)-3-酮-α-紫罗兰醇-9-O-β-D-葡萄吡喃糖苷[(6S,9R)-3-oxo-α-ionol-9-O-β-D-glucopyranoside,Ⅲ]、香鳞毛蕨苷a(fragranoside a,Ⅳ)。结论化合物为新化合物,化合物Ⅰ~Ⅲ在鳞毛蕨属植物中均为首次发现。药理实验表明,化合物Ⅰ、Ⅲ具有显著的细胞毒活性。
[Key word]
[Abstract]
Objective To study the chemical components from Dryopteris fragrans and their biological activities.Methods By means of HPLC and other column chromatography,the chemical components were isolated from D.fragrans and their chemical structures were identified by spectral and chemical tests in accordance with their physicochemical characteristics.Furthermore,the cytotoxicity of the chemical components for Bel-7402,HCT-8,and A-549 cells was measured by MTT method.Results Four compounds,geniposide(Ⅰ),3S,5R,6R,7E,9S-megastigman-7-ene-3,5,6,9-tetrol-3-O-β-D-glucopyranoside(Ⅱ),(6S,9R)-3-oxo-α-ionol-9-O-β-D-glucopyranoside(Ⅲ),and fragranoside a(Ⅳ) were iso-lated and identified.Conclusion Compound Ⅳ is a new compound.And all the other three compounds Ⅰ-Ⅲ are found in plants of Dryopteris Adanson for the first time.The Results of pharmacological experiments indicate that compounds Ⅰ and Ⅲ show strong cytotoxicity.
[中图分类号]
[基金项目]
黑龙江省自然科学基金资助项目(D2005-51);哈尔滨市重点科技攻关计划项目(GJ2007GH003003)