目的探讨葛根素防治慢性低氧高二氧化碳性肺动脉高压大鼠右心室肥大的效应是否与新的小分子活性肽Apelin及其受体(ApJ)有关。方法清洁级SD雄性大鼠30只,随机分为对照组、低氧高二氧化碳模型组和葛根素组。放免法测定血浆与右心室组织匀浆的Apelin-36水平,RT-pCR检测右心室组织Apelin与ApJ mRNA表达。结果右心室与左心室加室间隔的质量比[RV/(LV+S)]:模型组明显高于对照组(p<0.01),而葛根素组明显低于模型组(p<0.01);平均肺动脉压(mpAp):模型组明显高于对照组(p<0.01),而葛根素组明显低于模型组(p<0.05),平均颈动脉压(mCAp)各组间比较无显著统计学意义(p>0.05)。血浆Apelin-36水平:模型组明显高于对照组(p<0.01).葛根素组又高于模型组(p<0.05)。右心室肌匀浆Apelin-36水平:模型组明显高于对照组(p<0.05),葛根素组又明显比模型组高(p<0.01)。右心室肌Apelin mRNA水平:模型组明显低于对照组(p<0.05),而葛根素组显著高于模型组(p<0.01)。右心室肌ApJ mRNA:模型组低于对照组(p<0.05),而葛根素组高于模型组(p<0.05)。结论葛根素防治低氧高二氧化碳性肺动脉高压大鼠右心室肥大的机制可能部分与调节Apelin/ApJ系统有关。

Objective To investigate whether the effect of puerarin on right ventricle hypertrophy of pulmonary hypertensive rats induced by chronic hypoxia-hypercapnia was related to new peptide Apelin or its receptor(APJ).Methods Thirty male Sprague-Dawley rats were randomly divided into three groups, they are control group,hypoxia-hypercapnia 4-week model group,and hypoxia-hypercapnia 4-week plus puerarin group.The concentrations of Apelin-36 protein in plasma and homogenate of right ventricular muscle were detected by radioimmunoassay.The mRNA expressions of Apelin and APJ in right ventricu- lar muscle were measured by semi-quantitive reverse transcription polymerase chain reaction(RT-PCR). Results The weight ratio of right ventricle to left ventricle plus septum[RV/(LV+S)] in model group was significantly higher than that in control group(p<0.01),but much lower in puerarin group than that in model group(p<0.01).The mean pulmonary arterial pressure(mPAP)in model group was markedly higher than that in control group(p<0.01),but much lower in puerarin group than that in model group (p<0.05).There was no difference of mean carotid pressure(mCAP)(p<0.05).The plasma concen- tration of Apelin-36 protein in model group was significantly higher than that in control group(p<0.01), and obviously higher in puerarin group than that in model group(p<0.05).The concentration of Apelin- 36 protein in right ventricular muscle in model group was increased as compared with that in control group (p<0.05),and obviously higher in puerarin group than that in model group(p<0.01).The expression of Apelin mRNA in right ventricle in model group was markedly lower than that in control group(p<0.05),but significantly higher in puerarin group than that in model group(p<0.01).The expression of APJ mRNA in right ventricle was lower than that in control group(p<0.05),but higher in puerarin group than that in model group(p<0.05).Conclusion The mechanisms of puerarin in decreasing pul- monary hypertension and inhibiting right ventricle hypertrophy in rats chronically exposed to hypoxia-hy- percapnia are partly associated with regulating Apelin/APJ system.

浙江省自然科学基金(206464)