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[摘要]
目的建立铁皮石斛药材的RP-HPLC指纹图谱。方法采用梯度洗脱的方法,以KromasilKR100-5C18(250mm×4.6mm,5μm)为分析用色谱柱,以乙腈-0.4%磷酸溶液为流动相建立了铁皮石斛药材的指纹图谱,并进行了相似度的计算。结果该方法可使铁皮石斛中各成分得到较好的分离,并根据检测结果确定了15个共有指纹峰。结论该指纹图谱检测方法简便、重现性好,可作为铁皮石斛药材质量控制的重要方法。
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[Abstract]
ObjectiveTo establish the fingerprint for the drystem in Dend robium candidum by RP2HPLC.MethodsKromasil○RKR10025 C18 column (250 mm ×416 mm , 5Lm ) was used with amixture of acetonit rile 2014% phosphoru sacid as mobile phase in a gradient mode and the data were analyzed with ″Computer Aided Similarity Evaluation″sotwareResultsThe chemical constituents of D candidum were optimally separated, among which 15 fingerprint peaks in common were confirmed ConclusionThis method is simple, accuratewith good reproducibility, and can be used specifically for the quality control of D candidum.
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