[关键词]
[摘要]
目的采用HPLC法建立江西枳壳药材指纹图谱。方法Eclipse XDB-C18柱(250mm×4.6mm,5μm),流动相为甲醇-0.1%磷酸水,采用梯度洗脱,以1.0mL/min的体积流量于320nm波长处进行检测。结果通过对江西省道地产区10批酸橙及香橙枳壳指纹图谱的分析,共检出13个分离度良好的共有峰;并进行了不同品种枳壳指纹图谱的分析。结论本方法可以用于枳壳药材质量控制,还可以作为研究枳壳种质资源的有效手段。
[Key word]
[Abstract]
Objective To set up the f ingerprint for Fructus Aurantii in Jiangxi Province by using HPLC.Methods The chromatographic separation was performed on an Eclipse XDB-C18 column(250 mm×4. 6 mm, 5Lm, Agilent ) with a mobile phase consisting of methanol-water-phosphoricacid as gradienteluent at the flow rate of 1.0 mL/ min. The U V detection wavelength was set at 320nm. ResultsBy the determination of the ten batches of C. aurantium ′X iucheng ′and C.aurantium ′X iangcheng′in authentic regions of Jiangxi Province, thirteen common peaks were detected. And fingerprint analysis of different species was carried out. ConclusionThis method can provide a scientific basis for the quality control of Fructus Aurantii and be a powerful mean of germplasm resources.
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[基金项目]
国家自然科学基金资助项目(30460156);国家“十五”攻关项目(2004BA721A23);江西省自然科学基金资助项目(340025)