目的分析雌雄栝楼植株内含物组成及量的差异,为早期性别鉴定提供依据。方法测定雌雄植株叶片生理生化指标并进行差异显著性分析,采用HPLC法分析雌雄植株叶片化学成分差异。结果雄株的光合色素,可溶性蛋白质的量,SOD、POD活性,DNA浓度高于雌株,其中光合色素的量、POD活性差异达显著性水平。雌株的MDA、可溶性糖、紫外吸收物的量高于雄株,其中紫外吸收物的量差异达显著性水平。HPLC分析结果显示雌雄植株叶片的出峰数目、时间、峰高等参数明显不同。结论栝楼雌雄植株中内含物的组成及量存在一定差异,紫外吸收物及HPLC特征峰可望作为性别鉴定的指标。

Objective To analyze the different composition and contents of inclusion in female and male Trichosanthes kirilowiilowii individuals and to provide some references for the early sex identification.Methods Physiological and biochemical indices in T.kirilowii leaves were determined and significant analysis of the difference was performed.The difference of chemical composition was analyzed using HPLC.Results The contents of photosynthetic pigments and soluble proteins,the activities of superoxide dismutase(SOD) and peroxidase(POD),and the DNA concentration were higher in male individuals than those in female ones.The difference in photosynthetic pigments content and POD activity reached a significant level.The female individuals possessed higher contents of malondiadehycle(MDA),soluble saccharide,and UV-absorbing compounds than those in male ones,and the difference in UV-absorbing compounds reached a significant level.HPLC Assay revealed obvious differences in the peaking frequency,time,and the peak height.Conclusion There are differences in the composition and contents of inclusions in male and female T.kirilowii individuals,and the UV-absorbing compounds and the characteristic HPLC peak could potentially be as indices of sex identification.

同济大学科技发展基金资助项目(2000219008)