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[摘要]
目的初步探讨新藤黄酸的体内外抗肿瘤作用。方法采用MTT方法观察新藤黄酸对多种肿瘤细胞的增殖抑制作用;采用4、8、16、32mg/kg剂量新藤黄酸作用于人肿瘤裸小鼠(BALB/c-nude)模型,观察新藤黄酸的体内抗肿瘤作用。结果MTT法显示新藤黄酸对培养的人肿瘤细胞(人结肠癌细胞HCT-8、人肝癌细胞Bel-7402、人胃癌细胞BGC-823、人非小细胞肺癌细胞A549、人卵巢癌细胞A2780)增殖有一定的抑制作用,作用72h的IC50在1.75~3μmol/L;8、16、32mg/kg新藤黄酸iv给药,对人非小细胞肺癌A549细胞肿瘤移植的模型小鼠有一定的抑制肿瘤增长作用(P<0.05)。但4~16mg/kg剂量的新藤黄酸iv给药,对人肝癌Bel-7402肿瘤模型的抑制生长作用不明显。结论新藤黄酸能够抑制培养的肿瘤细胞生长;iv给药时对A549细胞肿瘤移植的模型小鼠肿瘤增殖有明显的抑制作用。
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[Abstract]
Objective To investigate the antitumor effects of neogambogic acid both in vitro and in vitro.Methods Cancer cell lines were incubated with various concentrations of neogambogic acid for 24,48,and 72 h,antiproliferative activities against cancer cells were measured by MTT assay;The BALB/c-nude mice were transplanted with cancer cells,then iv by 4,8,16,and 32 mg/kg neogambogic acid,the size and weight of the tumor were measured.Results Neogambogic acid could inhibit the proliferation of cancer cell lines(human colon carcinoma HCT-8 cell,human hepatoma Bel-7402 cell,human gastric carcinoma BGC-823 cell,human non-small cell lung cancer A549 cell,and human ovarian cancer A2780 cell).The 50% inhibiting concentration(IC50) of neogambogic acid of 72 h was from 1.75 to 3 μmol/L.It also showed the inhibition on the growth of A549 in nude mice at the doses of 8,16,and 32 mg/kg of neogambogic acid intravenously(P<0.05).However neogambogic acid did not show the obvious inhibition on the growth of tumor Bel-7402 at the dose from 4 to 16 mg/kg.Conclusion It suggests that neogambogic acid has the inhibitory effects on the proliferation of human cancer cell lines in vitro and on the tumor growth of nude mice transplanted with A549 cell in vitro.
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[基金项目]
安徽省自然科学基金资助项目(070413128);安徽省科技攻关重点项目(06013131B)