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[摘要]
目的对盾叶薯蓣进行化学成分研究。方法利用反相色谱技术分离盾叶薯蓣水相部分中的化学成分,用1H-NMR、13C-NMR、135DEPT、HMQC和HMBC等方法鉴定其结构。结果从新鲜的盾叶薯蓣根茎分得3个甾体皂苷,分别鉴定为薯蓣皂苷元-3-O-β-D-吡喃葡萄糖基(1→3)-β-D-吡喃葡萄糖基(1→4)-[α-L-吡喃鼠李糖基(1→2 )]-β-D-吡喃葡萄糖苷(Ⅰ)、(25R)-26-O-β-D-吡喃葡萄糖基-呋甾烷-5-烯-3β,22ζ-二醇-3-O-β-D-吡喃葡萄糖基(1→3)-β-D-吡喃葡萄糖基(1→4)-[α-L-吡喃鼠李糖基(1→2)]-β-D-吡喃葡萄糖苷(Ⅱ)、(25R)-26-O-β-D-吡喃葡萄糖基-呋甾烷-5-烯-3β,22ζ-二醇-7-羰基-3-O-β-D-吡喃葡萄糖基(1→3)-β-D-吡喃葡萄糖基(1→4)-[α-L-吡喃鼠李糖基(1→2)]-β-D-吡喃葡萄糖苷(Ⅲ)。结论化合物Ⅲ为一新甾体皂苷,命名为盾叶薯蓣皂苷H(zingiberenin H)。
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[Abstract]
Objective To study the chemical constituents of Dioscorea zingiberensis.Methods The fresh rhizome of D.zingiberensis was extracted three times,3 h once with EtOH-H2O at 80 ℃.The EtOH was evaporated under reduced pressure to give a residue which was suspended in water and then was exacted with petroleum ether,ethyl acetate,and n-butanol fraction.The water fraction was isolated by the reverseD-phase ODS column chromatography.The chemical structures were elucidated by means of 1H-NMR,13C-NMR,135DEPT,HMQC,and HMBC spectroscopic analyses.Results Three steroidal saponins were isolated from the fresh rhizome of D.zingiberensis.The compounds were identified as:diosgenin-3-O-β-D-glucopyranosyl(1→3)-β-D-glucopyranosyl(1→4)-[α-L-rhamnopyranosyl(1→2)]-β-D-glucopyranoside(Ⅰ),(25R)-26-O-β-D-glucopyranosyL-furost-5-en-3β,22ζ-dioL-3-O-β-D-glucopyranosyl(1→3)-β-D-glucopyranosyl(1→4)-[α-L-rhamnopyranosyl(1→2)]-β-D-glucopyranoside(Ⅱ),and(25R)-26-O-β-D-glucopyranosyL-furost-5-en-3β,22ζ-dioL-7-carbonyL-3-O-β-D-glucopyranosyl(1→3)-β-D-glucopyranosyl(1→4)-[α-L-rhamnopyranosyl(1→2)]-β-D-glucopyranoside(Ⅲ).Conclusion Compound Ⅲ is a novel compound named as zingiberenin H.
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