目的研究泡沫分离法分离重楼皂苷的半间歇式操作工艺。方法以富集比、回收率、纯度比为评价指标,对操作方式、气流速度、进料浓度、pH值、进料体积、温度进行单因素考察,研究其对泡沫分离效果的影响。结果在气流速度为400mL/min,进料质量浓度约为0.3mg/mL,pH7,液相高度维持在30cm,温度为40℃时,重楼皂苷泡沫分离的富集比为25.7,回收率为42.1%,泡沫液总皂苷纯度为41.4%,相比原料液纯度提高4.5倍。结论泡沫分离技术可较好地用于重楼中皂苷的分离。

Objective To study the process of foam fractionation of polyphyllin in semi-batch mode. Methods Taking enrichment ratio, recovery rate of polyphyllin, and purity ratio as the performance criteria and using single examining method to examine the operational parameters, i.e. operation mode, air flow rate, initial feed concentration, solution pH value, initial feed height and temperature on separation performance. The optimal conditions of the process were obtained finally. Results The separation performance is good when gas flow rate is 400 mL/min, initial feed concentration (polyphyllins content) is 0.3 mg/mL, pH value is 7, feed height is 30 cm, and feed temperature is 40 ℃. The enrichment ratio is 25.7, recovery ratio is 42.1%, and the foam liquids purity of total polyphyllin is 41.4%, which is 4.5 times higher than that in feed purity. Conclusion Foam fractionation technique could be applied to separate polyphyllin.

国家“十五”科技攻关重点项目(2004BA721A43)