[关键词]
[摘要]
目的 优选注射用红花提取物的纯化方法。方法 以红花黄色素(safflor yellow,SY)或羟基红花黄色素A(hydroxy safflor yellow A,HSYA)的转移率、质量分数,杂质的清除效果和复溶性作为考察指标,采用明胶沉淀法、澄清剂法、石硫醇法、碱性醇沉法和大孔吸附树脂法进行纯化,优选纯化条件,并对纯化结果进行比较。结果 采用大孔吸附树脂纯化红花提取物,HSYA的转移率及纯度较高,杂质清除效果较好,复溶性较好。结论 通过对不同纯化方法的比较,大孔吸附树脂法可提高红花提取物的纯化效果,优于其他纯化方法。
[Key word]
[Abstract]
Objective Optimizing the purification method for the injective extract from Carthamus tinc— toHus.Methods Taking the transfering rate,content,scavenging effect of impurities,and reconstitution of safflower yellow(SY)or hydroxysafflor yellow A(HSYA)as the indexes to optimize the effects of glutin precipitation,ZTCl+1,SHILIU elcohol,basic precipitation,and macroreticular resin method for purification and tO compare the results of the purification.Results When using macroreticular resin to pu— rify the extract of C.tinctorius,the transfering rate and purity of HSYA were much higher.The scaveng— ing effect of impurities and reconstitution were both good.Conclusion By comparison of different purifi— cation methods,it is found that the method using macroreticular resin could improve the purification and has a higher efficiency than others.
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[基金项目]
国家自然科学基金资助项目(30200363);辽宁省博士启动基金资助项目(2001102042)