目的对泻心汤不同配伍抗炎作用进行研究,以阐明该方的配伍原理。方法采用脂多糖(LPS)小鼠急性炎症模型,以血清NO浓度变化作为炎症指标,观察ig泻心汤生药18g/kg对血清NO浓度经时变化的影响;ig泻心汤生药4.5、9、18g/kg对血清NO浓度的影响;按正交设计,观察ig泻心汤不同配伍,即大黄、黄连、黄芩、大黄+黄连、大黄+黄芩、黄连+黄芩、全方(全方组生药18g/kg,其余各组按照在全方中的等量生药剂量给药)对血清NO浓度的影响。结果泻心汤给药后小鼠血清NO浓度均较对应时间点模型组低,6h差异显著,12、15h差异非常显著;泻心汤各剂量组小鼠血清NO浓度均较模型组低,高剂量组差异显著;不同配伍结果显示,除黄连组外,各给药组血清NO浓度与模型组相比差异显著;正交分析表明,在抗炎方面,大黄为该方第一要药,黄芩其次,黄芩与大黄有协同作用,黄连无明显作用及影响。结论在抗炎方面,泻心汤中大黄为第一要药,黄芩其次,黄芩与大黄有协同作用。

Objective To comparatively study the anti-inflammatory effects of ig Xiexin Decoction in various compatibilities in order to uncover the priciple of the compatibilities for this decoction. Methods Lipopolysaccharide (LPS)-induced acute inflammatory mouse model was adopted, nitric oxide (NO) concentration in serum was detected as an index of inflammation, the effect of ig Xiexin Decoction (18 g/kg) on the time course change of serum NO concentration of LPS-challenged mice and the effect of different dosages of ig Xiexin Decoction (4.5, 9, and 18 g/kg) on the serum NO concentration of LPS-challenged mice were checked; Comparative study on the effects of Xiexin Decoction and its components (Radix et Rhizoma Rhei, Rhizoma Coptidis, Radix Scutellariae, Radix et Rhizoma Rhei plus Rhizoma Coptidis, Radix et Rhizoma Rhei plus Radix Scutellariae, Rhizoma Coptidis plus Radix Scutellariae) on serum NO concentration of LPS-challenged mice was performed according to the principle of orthogonal design. The dosage of Xiexin Decoction was 18 g/kg, the dosages of its components were determined according to their proportions in the decoction. Results At every time point, NO concentration in the Xiexin Decoction group was lower than that in the model group, and significant difference was found at 6 h and very significant difference was found at 12 and 15 h. NO concentration of every groups treated with different dosages in Xiexin Decoction was lower than that in the model group, significant difference was found in the high dosage group. NO concentration of every groups treated with any component in Xiexin Decoction or the combination of two or three of them was lower than that in the model group, and significant difference was not found in the only Rhizoma Coptidis group. Variance analysis showed that as far as anti-inflammatory effect was concerned, Radix et Rhizoma Rhei was the most important component in this decoction, followed by Radix Scutellariae, while Rhizoma Coptidis was unessential. Variance analysis also showed that synergy was found between Radix et Rhizoma Rhei and Radix Scutellariae. Conclusion As far as anti-inflammatory effect is concerned, Radix et Rhizome Rhei is the most important component in Xiexin Decoction, followed by Radix Scutellaride, and there is a synergy between Radix et Rhizoma Rhei and Radix Scutellariae.

国家自然科学基金(90409008)；上海市科委基金(04DZ19844)；上海市重点学科建设项目资助(T0301)