目的 降低怀地黄试管苗的生产成本,为工厂化生产提供理论依据。方法 比较1/2MS和MS、自来水和蒸馏水、白砂糖和蔗糖对怀地黄试管苗生长发育的影响。结果 1/2MS培养基和MS培养基对怀地黄试管苗根数、鲜质量、干质量和叶绿素的量有极显著影响,对叶片数有显著影响,1/2MS培养基明显优于MS培养基。自来水和蒸馏水对试管苗根部、冠部、鲜质量增量、干质量和叶绿素的量均有显著性影响,试管苗在添加蒸馏水的培养基中的生长状况明显优于添加自来水。白砂糖和蔗糖对试管苗根部、冠部、鲜质量增量和干质量的影响不大,但对叶绿素的量有极显著影响,选用蔗糖作为碳源时,其叶片的叶绿素的量更高。结论 1/2MS可以取代MS,白砂糖可以取代蔗糖。

Objective To decrease the cost of plantlets of Rehmannia glutinosa f. huechingensis and pave the way for large scale production. Methods To compared the effects between 1/2 MS and MS, tap water and distilled water, table sugar and sucrose on the growth and development of R. glutinosa f. huaichingensis. Results (1) There was a significant difference in the number of roots, fresh weight, dry weight, and the chlorophyll content and a difference in the number of leaves between MS and 1/2 MS. So 1/2 MS is better than MS. (2) There was a significant difference in the root, the crown, the fresh weight increase, and the dry weight and the chlorophyll content between tap water and distilled water. By observation, the plant can grow better in distilled water. (3) There was a significant impact on the chlorophyll content between table sugar and sucrose, the content of chlorophyll in the medium of sucrose was higher, while little effect on root, crown, fresh weight increase, and dry weight. Conclusion So on a large scale of production, MS could be taken place with 1/2 MS and sucrose with table sugar.

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