目的 探讨赤芍总苷对HepA肝癌小鼠肿瘤生长及肿瘤细胞凋亡的影响及其机制。方法 60只健康昆明小鼠,随机分5组,分别为模型组,环磷酰胺(100mg/kg)组,赤芍总苷(240、120mg/kg)组,环磷酰胺(100mg/kg)+赤芍总苷(240mg/kg)组。复制小鼠HepA肝癌皮下移植模型后,第2天ig给药,连续给药7d,第8天处死,取肿瘤组织,计算抑瘤率,流式细胞仪检测肿瘤细胞凋亡率和分析细胞周期,以及免疫组化法检测凋亡相关蛋白Bcl-2和Bax的表达。结果 赤芍总苷高、低剂量明显抑制HepA肝癌小鼠肿瘤的生长;肿瘤细胞凋亡指数增加;抑制肿瘤细胞G₀/G₁期比例,向S期细胞转化,凋亡率增高;下调肿瘤细胞中Bcl-2蛋白的表达,同时明显提高肿瘤细胞中Bax蛋白的表达。结论 赤芍总苷对HepA肝癌小鼠肿瘤生长有明显抑制作用,并诱导肿瘤细胞凋亡,其主要机制与调节Bcl-2和Bax蛋白表达有关。

Objective To investigate the inhibitory effect of the total glucosides from Radix Paeoniae Rubra on the growth and apoptosis of hepatoma cells HepA and its mechanism. Methods KM Mice (60) inoculated with hepatoma cells were randomly divided into five groups: the model group, the cyclophoshamide (CY, 100 mg/kg) group, the groups with high- and low-doses (240 and 120 mg/kg) of the total glucosides from Radix Paeoniae Rubra the group with combination of CY (100 mg/kg) and high-dose (240 mg/kg) of the total glucosides from Radix Paeoniae Rubra, ig administration was given to the mice in day 2, respectively. The administration lasted 7 d, then the mice were sacrificed at day 8 and the tumor tissue was weighed. The inhibitory rates were calculated by weighing the tumor separately; The flow cytometer (FCM) was used to determine the apoptosis rates of hepatoma cells; The expression levels of Bcl-2 and Bax of the hepatoma cells were assayed by immunohistochemistry. Results The hepatoma cells in both high- and low-doses groups with the total glucosides from Radix Paeoniae Rubra were inhibited in a significant difference compared with the model group; It is showed that the larger dosage of the total glucosides from Radix Paeoniae Rubra is, the higher inhibitory rates are; The proportion in G₀/G₁ period cells could be inhibited and made to transfer to S period cell and increase the apoptosis rates by the total glucosides from Radix Paeoniae Rubra; The expression levels of Bcl-2 could be decreased while the expression levels of Bax in hepatoma cells could be increased by the total glucosides from Radix Paeoniae Rubra. Conclusion The total glucosides from Radix Paeoniae Rubra can inhibit the growth of hepatoma cells significantly and induce the apoptosis of hepatoma cells, which may be closely related with regulating the expression levels of Bcl-2 and Bax.

国家自然科学基金资助(305404200032,30510403202);长江学者和创新团队发展计划资助(IRT0413);博士点基金资助项目(20050026012);齐齐哈尔市科学技术计划重点项目