目的 建立测定泻心汤中芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚游离与结合型的法,并探索配伍对各成分的影响。方法 采用AgilentHypersilODS色谱柱(250mm×4.6mm,5μm);流动相:甲醇-0.1%磷酸(75∶25);体积流量:1.0mL/min;柱温:30℃;检测波长:440nm,进样量:50μL。不同配伍中蒽醌类成分的差异采用SPSS软件进行方差分析。结果 建立了同时测定5种蒽醌类成分的方法,这5个成分线性关系良好,加样回收率稳定。与单味大黄相比,大黄-黄芩中5种蒽醌类成分增加;黄连-大黄中芦荟大黄素、大黄素、大黄酚的总量增加,而大黄酸和大黄素甲醚总量减少;泻心汤中大黄酸总量减少,大黄素、大黄酚和大黄素甲醚总量增加,5种蒽醌类成分总和增加。结论 本法准确可靠,重现性好,结果稳定,适合于泻心汤中蒽醌类成分的分析。并且不同配伍对泻心汤中蒽醌类成分有影响。

Objective To determine the contents of free andglycoside-combined anthraquinones namely aloe—emodin，rhein，emodin，ehrysophanol，and physcionin Xiexin Decoction．and to study the influence of compatibility on the contents of the above—mentioned five anthraquinones in the decoctions．Methods TheAgilent Hypersil ODS column (250 mm×4．6 mm，5μm)was used with the mobile phase being methanol一0．1% phosphoric acid (75:25)，flow rate 1．0 mL／min，the column temperature at 30℃，detecting wavelength at 440 nm，and sampling 50μL．The differences of the anthraquinones among all combinations were tested by one—way analysisof variance using SPSS software．Results The concentrations of free and glycoside—combined anthraquinones in different decoctions were determined with HPLC．The linearequations of five anthraquinones were established and their recoveries were stable．Compared to Radix et Rhizoma Rhei alone．the contents of five anthraquinones were tncreasedby addingPadix Scutellariae to the decoction．The contents of aloe—emodin，emodin，chrysophanol were increased and rhein, physcion were reducedby adding Rhizoma Coptidis to the decoction．The contents of rhein were reduced and emodin，chrysophanol, physcion，and iotal five anthraquinones were increased in Xiexin Decotion. Conelosion The methods are accurate and suitable to determine the contents of five anthraquinones in Xiexin Decoction with a desirable repeatability．The contents of the five anthraquinones are influenced by different compatibilities．

国家自然科学基金资助项目(90409008);上海市科委基金资助项目(04DZ19844);上海市重点学科建设项目资助(T0301)