目的 应用近红外(NIR)光谱技术快速分析红参乙醇回流提取过程。方法 采用比色法测定提取液样品的总皂苷质量浓度作为对照值,同时采集提取液样品的NIR光谱。运用正交信号校正算法消除光谱中的干扰信息,采用偏最小二乘回归法建立NIR光谱校正模型。结果 NIR光谱校正模型能够准确地预测红参提取过程总皂苷质量浓度。结论 NIR光谱技术可用于红参醇提过程快速分析。

Objective Near-infrared (NIR) spectroscopy was used as a fast analytical technique in the ethanol reflux-extraction process of red ginseng. Methods The NIR spectra of the extracting solution of red ginseng were obtained and the reference measurements of the active constituent in the extracting solution were performed by the colorimetric method. Firstly, the interference information in the spectra was detected by orthogonal signal correction (OSC) method. Then a calibration model between NIR spectra and reference measurements was established by partial least square regression. Results The results showed that the predictive accuracy of NIR calibration model used for the determination of ginsenoside in ethanol extracting process of red ginseng was good. Conclusion NIR Spectroscopy could be applied to the fast analysis for ethanol extracting processes of red ginseng.

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