目的 建立阳春砂仁HPLC指纹图谱分析方法,初步拟定广东省阳春产阳春砂仁甲醇提取物指纹特征图谱指标成分群,为阳春砂仁药材内在质量评价积累数据。方法 采用Nucleodur C18 Gravity色谱柱;流动相为甲醇-2%乙酸(梯度洗脱);检测波长为260nm;柱温为30℃;体积流量1.0mL/min。结果 共标示出20个共有峰,18批阳春砂仁指纹图谱相似度较高。结论 方法准确可靠,重现性好,为阳春砂仁质量控制提供了参考。

Objective To establish the method of fingerprint analysis on the fruits of Amomum villosum by HPLC and work out the characteristic fingerprint of methanol extracts in the fruits of A.villosum from Yangchun,Guangdong Province as index component which could be used for the accumulation of data to evaluate the inner quality of the fruits of A.villosum.Methods HPLC with Nucleodur C18 Gravity column was used,the methanol-2% acetic acid(gradient elution)as a mobile phase,detection wavelength at 260 nm,column temperature was 30 ℃,and flow rate was 1.0 mL/min.Results Common peaks(20)were separated on HPLC fingerprint in A.villosum.The similarities of 18 batches of samples were higher.Conclusion The method is reliable and accurate,has a better reproducibility,and provides a reference for the quality control to the fruits of A.villosum.

广东省科技计划项目(2005B33001038)