[关键词]
[摘要]
目的 研究朝鲜淫羊藿Epimedium koreanum的非黄酮类化学成分。方法 采用硅胶、SephadexLH-20、ODS柱色谱分离朝鲜淫羊藿干燥地上部分的非黄酮类化学成分;应用物理化学方法及1D和2DNMR方法分析确定化学结构;用MTT法检测化合物对大鼠骨肉瘤UMR106细胞增殖的影响。结果 从朝鲜淫羊藿水提取物中分离得到2个倍半萜类化合物和2个9,10-二氢菲类化合物,分别鉴定为:3,7,11-三甲基-2,6-十二二烯-1,10,11-三羟基-10(S)-O-β-D-吡喃葡萄糖苷(3,7,11-trimethyl-2,6-dodecadien-1,10,11-trihydroxy-10(S)-O-β-D-glucopyranoside,Ⅰ)、淫羊藿苷C1(icarisideC1,Ⅱ)、淫羊藿苷A5(icarisideA5,Ⅲ)、epimedoicarisosideA(Ⅳ)。结论 化合物Ⅰ为新化合物,命名为淫羊藿苷F(icarisideF),化合物Ⅲ为首次从该种植物中分离得到;化合物Ⅱ~Ⅳ有促进UMR106细胞增殖的作用。
[Key word]
[Abstract]
Objective To study the nonflavanoid constituents in the aerial part of Epimedium koreanum.Methods The constituents were isolated through column chromatography on silica gel,Sephadex LH-20,and ODS from the water extract of E.koreanum,and their structures were identified by physicochemical methods and spectroscopic analysis.MTT Method was used to study the effects of the cons-tituents on the proliferation of UMR106 cell in rats.Results Two sesquiterpenes and two 9,10-dihydrophenanthrene derivatives were isolated from the water extract of E.koreanum.Their structures were identified as 3,7,11-trimethyl-2,6-dodecadien-1,10,11-trihydroxyl-10(S)-O-β-D-glucopyranoside(Ⅰ),icariside C1(Ⅱ),icariside A5(Ⅲ),epimedoicarisoside A(Ⅳ).Conclusion Compound Ⅰ is a new compound,named icariside F.Compound Ⅲ is isolated from E.koreanum for the first time.Compounds Ⅱ-Ⅳ show the proliferation-promoting activities on UMR106 cell.
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[基金项目]
国家自然科学基金委员会与香港研究资助局联合基金资助项目(30418007)